Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Absolutt kvantifisering av Cell-fri protein…
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content.

JoVE Journal Biochemistry
Absolutt kvantifisering av Cell-fri protein syntese metabolisme av reversert-fase flytende kromatografi-Mass massespektrometri
Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
Journal (Biochemistry)
Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation
Journal (Biochemistry)
A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation
 
Click here for the English version

Absolutt kvantifisering av Cell-fri protein syntese metabolisme av reversert-fase flytende kromatografi-Mass massespektrometri

Article DOI: 10.3791/60329-v • 08:06 min • October 25th, 2019
October 25th, 2019 •
1, 1, *1, *1, 1
1Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Her presenterer vi en robust protokoll for å kvantifisere 40 forbindelser involvert i Sentral karbon og energi metabolisme i celle-fri protein syntese reaksjoner. Den celle frie syntese blandingen er derivatized med anilin for effektiv separasjon ved hjelp av reversert-fase flytende kromatografi og deretter kvantifisert av masse massespektrometri bruker isotopically merket interne standarder.

Tags

Biokjemi celle-fri proteinsyntese flytende kromatografi-masse massespektrometri anilin merking sentral karbon energi metabolisme intern standard metabolske nettverk isotopanrikning merking
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter