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Samtidige flow Cytometrisk karakterisering af flere celletyper hentet fra mus hjerne/rygmarv gennem forskellige homogenisering metoder
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Samtidige flow Cytometrisk karakterisering af flere celletyper hentet fra mus hjerne/rygmarv gennem forskellige homogenisering metoder

Article DOI: 10.3791/60335-v • 10:24 min • November 19th, 2019
November 19th, 2019 •
*1, *1, 1,2, 1,2
1Dana-Farber/Boston Children's Cancer and Blood Disorders Center, 2Harvard Medical School
* These authors contributed equally

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Vi præsenterer en flow cytometri metode til at identificere samtidigt forskellige celletyper hentet fra mus hjerne eller rygmarv. Denne metode kan udnyttes til at isolere eller karakterisere rene cellepopulationer i neurodegenerative sygdomme eller til at kvantificere omfanget af celle målretning ved in vivo administration af virale vektorer eller nanopartikler.

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Medicin flow cytometry mus hjerne mus rygmarv vævshomogenisering microglia astrocytter oligodendrocytter endothelium neuroner
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