JoVE Journal
Developmental Biology
Developmental Biology
A subscription to JoVE is required to view this content.
半高通量成像方法和数据可视化工具包,用于分析C. elegans胚胎发育
The JoVE video player is compatible with HTML5 and Adobe Flash. Older browsers that do not support HTML5 and the H.264 video codec will still use a Flash-based video player. We recommend downloading the newest version of Flash here, but we support all versions 10 and above.
If that doesn't help, please let us know.
Unable to load video. Please check your Internet connection and reload this page. If the problem continues, please let us know and we'll try to help.
An unexpected error occurred. Please check your Internet connection and reload this page. If the problem continues, please let us know and we'll try to help.
Click here for the English version
Article
DOI:
10.3791/60362-v
•
06:49 min
•
October 29th, 2019
半高通量成像方法和数据可视化工具包,用于分析C. elegans胚胎发育
October 29th, 2019
•
Renat N. Khaliullin1,2,3, Jeffrey M. Hendel1,2, Adina Gerson-Gurwitz1,2, Shaohe Wang1,4,5, Stacy D. Ochoa1,6, Zhiling Zhao1,7, Arshad Desai1,2, Karen Oegema1,2, Rebecca A. Green1,2
1Ludwig Institute for Cancer Research, San Diego, 2Department of Cellular and Molecular Medicine, University of California, San Diego, 3Recursion Pharmaceuticals, 4Biomedical Sciences Graduate Program, University of California, San Diego, 5Cell Biology Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, 6Department of Biology, San Diego State University, 7Developmental and Stem Cell Biology Graduate Program, University of California, San Francisco
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
这项工作描述了一个半高通量协议,允许在一次一夜的运行中同时对80-100 C.elegans胚胎的胚胎发生进行3D延时成像。此外,还包括图像处理和可视化工具,以简化数据分析。这些方法与自定义报告菌株相结合,有助于详细监测胚胎生成。