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JoVE Journal Bioengineering
Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform
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Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform

Article DOI: 10.3791/60383-v • 08:10 min • October 6th, 2019
October 6th, 2019 •
1, 1,2,3
1Department of Biomedical Engineering, University of Delaware, 2Department of Materials Science & Engineering, University of Delaware, 3Delaware Biotechnology Institute

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This protocol provides instructions for implementing multiphoton lithography to fabricate three-dimensional arrays of fluorescent fiducial markers embedded in poly(ethylene glycol)-based hydrogels for use as reference-free, traction force microscopy platforms. Using these instructions, measurement of 3D material strain and calculation of cellular tractions is simplified to promote high-throughput traction force measurements.

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Traction Force Microscopy Reference-free Platform Cell-generated Forces Collection And Analysis Of Data Inexperienced Users Multiphoton Lithography Patterning Setting Redesign Fabrication And Implementation Visual Representation Photopolymerization Hydrogel Pre-polymer Solution PDMS Strips Coverslip UV Light Exposure Petri Dish
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