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Journal / Bioengineering / Trasduzione ed espansione delle cellule T…
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Bioengineering

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JoVE Journal Bioengineering
Trasduzione ed espansione delle cellule T primarie in nove giorni con manutenzione del fenotipo della memoria centrale
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Trasduzione ed espansione delle cellule T primarie in nove giorni con manutenzione del fenotipo della memoria centrale

Article DOI: 10.3791/60400-v • 08:49 min • March 18th, 2020
March 18th, 2020 •
1, 1
1Department of Veterinary and Biomedical Sciences, University of Minnesota

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Illustreremo un protocollo di 9 giorni per la trasduzione e l'espansione delle cellule mononucleari periferiche del macaco rhesus che produce cellule con un'eccellente co-espressione dei geni di interesse in numero sufficiente per gli studi di infusione dell'efficacia cellulare.

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Bioingegneria Numero 157 Cellule CAR-T trasduzione espansione retrovirus PBMC memoria centrale
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