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Journal / Cancer Research / Isolatie en uitbreiding van cytotoxische…
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JoVE Journal Cancer Research
Isolatie en uitbreiding van cytotoxische Cytokine-geïnduceerde Killer T-cellen voor kankerbehandeling
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Isolatie en uitbreiding van cytotoxische Cytokine-geïnduceerde Killer T-cellen voor kankerbehandeling

Article DOI: 10.3791/60420-v • 11:11 min • January 24th, 2020
January 24th, 2020 •
1,2,3, 4, 4,5, 4,6,7, 1,2, 1,2, 4, 4
1Department of Urology, Wan Fang Hospital, 2Department of Urology, School of Medicine, College of Medicine, Taipei Medical University, 3Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, 4Translational Cell Therapy Center, Department of Medical Research, China Medical University Hospital, 5Graduate Institute of Biomedical Sciences, China Medical University, 6Graduate Institute of Immunology, China Medical University, 7Department of Neurosurgery, Neuropsychiatric Center, China Medical University Hospital

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Hier presenteren we een protocol om de isolatie en uitbreiding van perifere bloedmononucleaire cellen-afgeleide cytokine-geïnduceerde CD3+CD56+ killer cellen uit te voeren en hun cytotoxiciteitseffect tegen hematologische en vaste kankercellen te illustreren met behulp van een in vitro diagnosestroom cytometriesysteem.

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Cancer Research adoptieve cellulaire immunotherapie cytokine-geïnduceerde killer cel perifere bloed mononucleaire cellen hematologische en vaste kankers immunocellulaire therapie cytotototoxische effect stroom cytometrie
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