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JoVE Journal Cancer Research
Isolamento ed espansione delle cellule T Killer citotokine indotte da citokine per il trattamento del cancro
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Isolamento ed espansione delle cellule T Killer citotokine indotte da citokine per il trattamento del cancro

Article DOI: 10.3791/60420-v • 11:11 min • January 24th, 2020
January 24th, 2020 •
1,2,3, 4, 4,5, 4,6,7, 1,2, 1,2, 4, 4
1Department of Urology, Wan Fang Hospital, 2Department of Urology, School of Medicine, College of Medicine, Taipei Medical University, 3Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, 4Translational Cell Therapy Center, Department of Medical Research, China Medical University Hospital, 5Graduate Institute of Biomedical Sciences, China Medical University, 6Graduate Institute of Immunology, China Medical University, 7Department of Neurosurgery, Neuropsychiatric Center, China Medical University Hospital

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Qui, presentiamo un protocollo per eseguire l'isolamento e l'espansione delle cellule mononucleari periferiche derivate dalle cellule citochine- indotte CD3-CD56- cellule killer e illustrare il loro effetto di citotossicità contro le cellule tumorali ematologiche e solide utilizzando un sistema di citometria di flusso di diagnosi invitro.

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Ricerca sul cancro numero 155 immunoterapia cellulare adottiva cellula killer indotta da citochine cellule mononucleari del sangue periferico tumori ematologici e solidi immunocellulare terapia effetto citotossico citometria a flusso
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