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Journal / Immunology and Infection / Análise do Grupo IV Viral SSHHPS usando métodos…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Análise do Grupo IV Viral SSHHPS usando métodos in vitro e em silico
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Análise do Grupo IV Viral SSHHPS usando métodos in vitro e em silico

Article DOI: 10.3791/60421-v • 10:40 min • December 21st, 2019
December 21st, 2019 •
1, 2, 2
1National Center for Advancing Translational Sciences, National Institutes of Health, 2United States Naval Research Laboratory

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Apresentamos um protocolo geral para identificar trechos curtos de sequências de proteínas homologous hospedeiras-patógenos (SSHHPS) incorporadas na poliproteína viral. SSHHPS são reconhecidos por proteases virais e direcionam a destruição direcionada de proteínas hospedeiras específicas por vários vírus do Grupo IV.

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Imunologia e Infecção Edição 154 enzima ensaio FRET proteína não estrutural alfavírus Grupo IV protease SSHHPS ensaio de gel in vitro acoplamento
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