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Journal / Cancer Research / Subcellulaire fractionering van primaire…
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Cancer Research

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JoVE Journal Cancer Research
Subcellulaire fractionering van primaire chronische lymfatische leukemie cellen om te controleren van nucleaire/cytoplasmatische eiwit handel
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Subcellulaire fractionering van primaire chronische lymfatische leukemie cellen om te controleren van nucleaire/cytoplasmatische eiwit handel

Article DOI: 10.3791/60426-v • 11:39 min • October 23rd, 2019
October 23rd, 2019 •
*1, *1, 1, 1
1Paul O'Gorman Leukaemia Research Centre, Institute of Cancer Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow
* These authors contributed equally

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Dit protocol maakt de optimalisatie en de daaropvolgende efficiënte generatie van nucleaire en cytoplasmatische fracties van primaire chronische lymfatische leukemie cellen. Deze monsters worden gebruikt om te bepalen van de eiwit lokalisatie, alsmede veranderingen in de handel in eiwitten die plaatsvinden tussen de nucleaire en cytoplasmische compartimenten op celstimulatie en medicamenteuze behandeling.

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Kankeronderzoek probleem 152 nucleaire/cytoplasmatische fractionering chronische lymfatische leukemie cellulaire signalering medicamenteuze behandeling FOXO eiwit handel
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