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JoVE Journal Neuroscience
Isolation og kultur af oculomotor, Trochlear, og spinal motor neuroner fra prænatal ISLMN
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Isolation og kultur af oculomotor, Trochlear, og spinal motor neuroner fra prænatal ISLMN: gfp Transgene mus

Article DOI: 10.3791/60440-v • 10:04 min • November 12th, 2019
November 12th, 2019 •
1,2,3,4,9, 1,2,10, 1,2,3,7, 2,5,6, 1,2,3,4,5,6,7,8
1Department of Neurology, Boston Children's Hospital, 2FM Kirby Neurobiology Center, Boston Children's Hospital, 3Department of Neurology, Harvard Medical School, 4Medical Genetics Training Program, Harvard Medical School, 5Department of Ophthalmology, Boston Children's Hospital, 6Department of Ophthalmology, Harvard Medical School, 7Broad Institute of M.I.T. and Harvard, 8Howard Hughes Medical Institute, 9Department of Neurology, Kokura Memorial Hospital, 10Department of Genetics, Albert Einstein College of Medicine

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Dette arbejde præsenterer en protokol til at give homogene cellekulturer af primære oculomotor, trochlear, og spinal motoriske neuroner. Disse kulturer kan anvendes til komparative analyser af de morfologiske, cellulære, molekylære og elektrofysiologiske egenskaber af okulære og spinal motoriske neuroner.

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Neurovidenskab motor neuron oculomotoriske Neuron trochlear Neuron primær kultur mus embryonale motor neuron kultur FACS ISLMN: gfp Transgene mus celle rensning celle isolation
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