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Journal / Neuroscience / Isolation und Kultur von Okulomotor, Trochlea,…
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Neuroscience

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JoVE Journal Neuroscience
Isolation und Kultur von Okulomotor, Trochlea, und Spinal Motor Neuronen von pränatalen Islmn
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Isolation und Kultur von Okulomotor, Trochlea, und Spinal Motor Neuronen von pränatalen Islmn:GFP Transgene Mäuse

Article DOI: 10.3791/60440-v • 10:04 min • November 12th, 2019
November 12th, 2019 •
1,2,3,4,9, 1,2,10, 1,2,3,7, 2,5,6, 1,2,3,4,5,6,7,8
1Department of Neurology, Boston Children's Hospital, 2FM Kirby Neurobiology Center, Boston Children's Hospital, 3Department of Neurology, Harvard Medical School, 4Medical Genetics Training Program, Harvard Medical School, 5Department of Ophthalmology, Boston Children's Hospital, 6Department of Ophthalmology, Harvard Medical School, 7Broad Institute of M.I.T. and Harvard, 8Howard Hughes Medical Institute, 9Department of Neurology, Kokura Memorial Hospital, 10Department of Genetics, Albert Einstein College of Medicine

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Diese Arbeit stellt ein Protokoll vor, um homogene Zellkulturen von primären okulomotorischen, Trochlea- und Spinalmotorneuronen zu ergeben. Diese Kulturen können für vergleichende Analysen der morphologischen, zellulären, molekularen und elektrophysiologischen Eigenschaften von augen- und spinalmotorischen Neuronen verwendet werden.

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Neurowissenschaften Ausgabe 153 Motorneuron okulomotorischer Neuron Trochlea-Neuron Primärkultur Maus embryonale motorische Neuronenkultur FACS IslMN:GFP transgene Maus Zellreinigung Zellisolation
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