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Journal / Neuroscience / Isolamento e coltura di Oculomotor, Trochlear, e…
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JoVE Journal Neuroscience
Isolamento e coltura di Oculomotor, Trochlear, e MotoNeuroni Spinali da Prenatal Islmn
Extraction and Dissection of the Domesticated Pig Brain
Journal (Neuroscience)
Extraction and Dissection of the Domesticated Pig Brain
Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections
Journal (Neuroscience)
Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections
 
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Isolamento e coltura di Oculomotor, Trochlear, e MotoNeuroni Spinali da Prenatal Islmn:GFP Topi Transgenici

Article DOI: 10.3791/60440-v • 10:04 min • November 12th, 2019
November 12th, 2019 •
1,2,3,4,9, 1,2,10, 1,2,3,7, 2,5,6, 1,2,3,4,5,6,7,8
1Department of Neurology, Boston Children's Hospital, 2FM Kirby Neurobiology Center, Boston Children's Hospital, 3Department of Neurology, Harvard Medical School, 4Medical Genetics Training Program, Harvard Medical School, 5Department of Ophthalmology, Boston Children's Hospital, 6Department of Ophthalmology, Harvard Medical School, 7Broad Institute of M.I.T. and Harvard, 8Howard Hughes Medical Institute, 9Department of Neurology, Kokura Memorial Hospital, 10Department of Genetics, Albert Einstein College of Medicine

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Questo lavoro presenta un protocollo per produrre colture cellulari omogenee dei motoneuroni primari oculomotori, trocleari e spinali. Queste colture possono essere utilizzate per analisi comparative delle caratteristiche morfologiche, cellulari, molecolari ed elettrofisiologiche dei motoneuroni oculari e spinali.

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Neuroscienze numero 153 motoneurone neurone oculomotore neurone trochlear coltura primaria coltura del motoneurone embrionale del topo FACS IslMN: Topo transgenico GFP purificazione cellulare isolamento cellulare
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