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Journal / Biology / 使用CyanoGate模块化克隆工具包进行共生对蓝藻的遗传改造…
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使用CyanoGate模块化克隆工具包进行共生对蓝藻的遗传改造

Article DOI: 10.3791/60451-v • 08:25 min • October 31st, 2019
October 31st, 2019 •
*1,2,3, *1,2, *1,2, 2,3, 1,2
1Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, 2Centre for Synthetic and Systems Biology, University of Edinburgh, 3Institute of Quantitative Biology, Biochemistry and Biotechnology, School of Biological Sciences, University of Edinburgh
* These authors contributed equally

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在这里，我们提出一个协议，描述如何使用CyanoGate模块化克隆工具包组装自我复制载体，ii） 通过结合将载体引入蓝藻宿主，iii） 使用板读取器或流式细胞仪。

Tags

生物学， 第 152 期， 流式细胞学， 荧光， 金门， 板读取器， Synechocystis sp. PCC 6803， Synechocous 拉隆UTEX 2973， 工具包， 瞬态表达
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