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Immunology and Infection

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JoVE Journal Immunology and Infection
Un essai de charge bactérienne moléculaire de la tuberculose (TB-MBLA)
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Un essai de charge bactérienne moléculaire de la tuberculose (TB-MBLA)

Article DOI: 10.3791/60460-v • 10:41 min • April 30th, 2020
April 30th, 2020 •
1, 1,2, 1, 1, 1, 3, 4, 1, 1
1Division of Infection and Global Health, School of Medicine, University of St Andrews, 2National Institute for Medical Research (NIMR)-Mbeya Medical Research Centre, 3Instituto Nacional de Saúde (INS), Ministério da Saúde, 4Institute of Biodiversity, Animal Health & Comparative Medicine, College of Medical, Veterinary & Life Sciences, University of Glasgow

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Nous décrivons un test d’essai moléculaire de charge bactérienne de la tuberculose effectué après l’inactivation de chaleur des expectorations. L’inactivation de la chaleur rend les échantillons d’expectorations non infectieux et évite la nécessité de laboratoires de confinement de niveau 3 pour les tests moléculaires de la tuberculose.

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Immunologie et infection Numéro 158 tuberculose Mycobacterium tuberculosis viabilité inactivation de la chaleur ARN analyse de charge bactérienne moléculaire transcriptase inverse quantitative PCR
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