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Journal / Biochemistry / Cell kultur på Silicon nitride membraner og…
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Biochemistry

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JoVE Journal Biochemistry
Cell kultur på Silicon nitride membraner og Cryopreparation for Synchrotron X-ray fluorescens nano-analyse
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Cell kultur på Silicon nitride membraner og Cryopreparation for Synchrotron X-ray fluorescens nano-analyse

Article DOI: 10.3791/60461-v • 08:26 min • December 10th, 2019
December 10th, 2019 •
1, 1, 2, 2, 1, 2, 1,2
1Inserm, UA7, Synchrotron Radiation for Biomedicine (STROBE), Université Grenoble Alpes, 2ID16A Beamline, ESRF, The European Synchrotron

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Presentert her er en protokoll for cellekultur på silisium nitride membraner og stupe-frysing før X-ray fluorescens Imaging med en Synchrotron kryogene X-ray hippocampusen. Når bare romtemperatur nano-analysen er gitt, kan de frosne prøvene bli ytterligere fryse-tørket. Dette er avgjørende skritt for å få informasjon om intracellulære Elemental sammensetning.

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Biokjemi kreftceller sporstoff Synchrotron stråling røntgen fluorescens mikroskopi cryofixation fryse-tørking hippocampusen
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