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Immunology and Infection

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JoVE Journal Immunology and Infection
Pseudomonas aeruginosa Küçük Koloni Varyant Kültürü ve Aljinat Quantitation
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Pseudomonas aeruginosa Küçük Koloni Varyant Kültürü ve Aljinat Quantitation

Article DOI: 10.3791/60466-v • 09:06 min • February 22nd, 2020
February 22nd, 2020 •
*1, *2, 1,2,3
1Department of Biomedical Sciences, Joan C. Edwards School of Medicine, Marshall University, 2Progenesis Technologies LLC, Robert C. Byrd Biotechnology Science Center, 3Department of Pediatrics, Joan C. Edwards School of Medicine, Marshall University
* These authors contributed equally

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Burada, pseudomonas aeruginosaküçük koloni varyantı kültüre bir büyüme durumu açıklar. Ayrıca p. aeruginosa tarafından üretilen eksopolisakkarit aljinatının saptanması ve nicelliği için geleneksel üronik asit karbazol testi ve aljinatspesifik monoklonal antikor (mAb) bazlı ELISA kullanılarak iki ayrı yöntem tanımlanmıştır.

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İmmünoloji ve Enfeksiyon Sayı 156 Pseudomonas aeruginosa,kistik fibrozis (CF) küçük koloni varyantı (SCV) pirimidin biyosentezi büyüme mücoidy aljinat üronik asit karbazol analizi aljinat spesifik mAb ELISA
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