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Cancer Research

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JoVE Journal Cancer Research
Cultura, manipolazione e trapianto ortotopico di organidi tumorali della vescica del topo
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Cultura, manipolazione e trapianto ortotopico di organidi tumorali della vescica del topo

Article DOI: 10.3791/60469-v • 08:43 min • January 31st, 2020
January 31st, 2020 •
*1, *1, *1, 1
1Department of Life Sciences, Pohang University of Science and Technology
* These authors contributed equally

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Questo protocollo fornisce passaggi sperimentali dettagliati per stabilire una coltura tridimensionale in vitro degli organoidi tumorali della vescica derivati dal cancro della miscite della vescica indotta da cancerogeni cancero. Sono descritti metodi di coltura tra cui passaggio, ingegneria genetica e trapianto ortotopico di organoidi tumorali.

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Ricerca sul cancro Problema 155 organoidi tumorali coltura 3D tumore della vescica carcinoma uteliale invasivo trasduzione lentivirale trapianto ortotopico
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