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JoVE Journal
Genetics

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使用酵母毒性和抑制器屏幕识别细菌效应蛋白的宿主通路
 
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使用酵母毒性和抑制器屏幕识别细菌效应蛋白的宿主通路

Article DOI: 10.3791/60488-v 07:40 min October 25th, 2019
October 25th, 2019

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

细菌病原体将蛋白质分泌到宿主中,以关键生物过程为目标。识别细菌效应蛋白所针对的宿主通路是解决分子发病机制的关键。这里,描述了一种使用改性酵母抑制剂和毒性筛分的方法,以阐明有毒细菌效应蛋白所针对的宿主通路。

Tags

遗传学, 问题 152, 细菌效应蛋白, 酵母抑制剂, 效应蛋白功能, 酵母屏风,衣原体沙眼,III型分泌效应蛋白, 通路鉴定
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