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Quantificação de Células Proliferativas e Mortas em Enteróides
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Quantificação de Células Proliferativas e Mortas em Enteróides

Article DOI: 10.3791/60501-v • 10:47 min • January 31st, 2020
January 31st, 2020 •
*1, *1, *1, 1, 1, 1, 1, 2, 2,3, 1, 1, 1
1Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genomic Resource Center, Medical College of Soochow University, Department of Oncology, The First Affiliated Hospital of Soochow University, 2Department of Pathology, University of Chicago, 3Department of Pathology, Brigham and Women's Hospital–Harvard Medical School
* These authors contributed equally

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O protocolo apresentado utiliza citometria de fluxo para quantificar o número de células proliferantes e mortas em enteróides de camundongos cultivados. Este método é útil para avaliar os efeitos do tratamento medicamentoso sobre a proliferação organoide e a sobrevivência.

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Biologia Edição 155 enteróides intestino citometria de fluxo proliferação EdU iodeto de propídio
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