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JoVE Journal Cancer Research
Kvantificering af tumorcellevedhæftning i lymfeknude Cryosektioner
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Kvantificering af tumorcellevedhæftning i lymfeknude Cryosektioner

Article DOI: 10.3791/60531-v • 06:09 min • February 9th, 2020
February 9th, 2020 •
1, 1, 1, 1
1Molecular Oncology Center, Hospital Sírio-Libanês

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Her beskriver vi en enkel og billig metode, der gør det muligt at kvantificere klæbende tumorceller til lymfeknude (LN) kryosektioner. LN-klæbende tumorceller er let identificeret ved lys mikroskopi og bekræftet af en fluorescens-baseret metode, hvilket giver en vedhæftning indeks, der afslører tumor celle-bindende affinitet til LN parenkym.

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Kræftforskning metastase lymfeknude kræftprogression cellevedhæfte integrinere ekstracellulære matrix
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