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Immunology and Infection

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JoVE Journal Immunology and Infection
Antibiotika-Dereplication ved hjælp af antibiotikaresistens platformen
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Antibiotika-Dereplication ved hjælp af antibiotikaresistens platformen

Article DOI: 10.3791/60536-v • 10:49 min • October 17th, 2019
October 17th, 2019 •
1, 2, 1
1Department of Biochemistry and Biomedical Sciences, M.G. DeGroote Institute for Infectious Disease Research, McMaster University, 2Department of Molecular and Cellular Biology, University of Guelph

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Vi beskriver en platform, der udnytter et bibliotek af isogene antibiotikaresistente Escherichia coli til dereplication af antibiotika. Identiteten af et antibiotikum produceret af bakterier eller svampe kan udledes af væksten af E. coli udtrykker sin respektive resistens gen. Denne platform er økonomisk effektiv og tidsbesparende.

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Immunologi og infektion antibiotika dereplication Drug Discovery actinomycetes Escherichia coli sekundær metabolit resistens enzymer naturprodukt
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