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Immunology and Infection

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Antibiotikum Dereplication med hjälp av antibiotikaresistens plattform
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Antibiotikum Dereplication med hjälp av antibiotikaresistens plattform

Article DOI: 10.3791/60536-v • 10:49 min • October 17th, 2019
October 17th, 2019 •
1, 2, 1
1Department of Biochemistry and Biomedical Sciences, M.G. DeGroote Institute for Infectious Disease Research, McMaster University, 2Department of Molecular and Cellular Biology, University of Guelph

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Vi beskriver en plattform som använder ett bibliotek av isogena antibiotikaresistenta Escherichia coli för dereplication av antibiotika. Identiteten hos ett antibiotikum som produceras av bakterier eller svampar kan härledas genom tillväxten av E. coli som uttrycker dess respektive motstånds gen. Denna plattform är ekonomiskt effektiv och tidseffektiv.

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Immunologi och infektion antibiotika dereplication Drug discovery aktinomyceter Escherichia coli sekundär metabolit resistensenzymer naturprodukt
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