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Nucleofection e In Vivo Propagação de Parasitas de Eimeria de Frango
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Nucleofection e In Vivo Propagação de Parasitas de Eimeria de Frango

Article DOI: 10.3791/60552-v • 08:26 min • February 14th, 2020
February 14th, 2020 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 2, 1, 1
1National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, 2School of Life Sciences, Peking University

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Aqui, fornecemos um método para alcançar a transfecção estável de parasitas de frango Eimeria por nucleofecting esporozoitas ou merozoitas de segunda geração. Parasitas eimerianos geneticamente modificados expressando genes antigênicos heterologous poderiam ser usados como veículos de entrega de vacinas.

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Biologia Edição 156 transfecção merozoitas esporozoitas Eimeria ferramenta inoculação
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