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Journal / Immunology and Infection / 在沙门氏菌感染组织培养细胞中，NF-βB依赖性路西酶的激活和基因表达的定量…
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Immunology and Infection

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JoVE Journal Immunology and Infection
在沙门氏菌感染组织培养细胞中，NF-βB依赖性路西酶的激活和基因表达的定量
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Intracellular Staining and Flow Cytometry to Identify Lymphocyte Subsets within Murine Aorta, Kidney and Lymph Nodes in a Model of Hypertension
Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells
Journal (Immunology and Infection)
Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells
 
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在沙门氏菌感染组织培养细胞中，NF-βB依赖性路西酶的激活和基因表达的定量

Article DOI: 10.3791/60567-v • 10:57 min • January 12th, 2020
January 12th, 2020 •
1, 1
1Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus

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在这里，我们提出了一个协议，通过测量细胞裂解中的发光，快速、轻松地测量活性B细胞（NF-B）激活细胞系中激活的核因子kappa-光链增强剂：：快星酶报告器结构。此外，基因表达通过从感染沙门氏菌的细胞中分离的RT-qPCR确定。

Tags

免疫学和感染，第155期，NF-βB，荧光素酶，发光，沙门氏菌，组织培养，免疫反应，基因表达，RT-qPCR
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