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Journal / Biology / 초구조적으로 보존된 세포 내 막 단백질 국소화의 전자 현미경 분석을 위한 CryoAPEX …
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초구조적으로 보존된 세포 내 막 단백질 국소화의 전자 현미경 분석을 위한 CryoAPEX 방법
Author Spotlight: Investigating the Relationship Between FSH and Pathophysiological Changes in Perimenopausal Women - Insights from a Mouse Model (Video) | JoVE
Journal (Biology)
Author Spotlight: Investigating the Relationship Between FSH and Pathophysiological Changes in Perimenopausal Women - Insights from a Mouse Model (Video) | JoVE
Isolation of Primary Rat Hepatocytes with Multiparameter Perfusion Control
Journal (Biology)
Isolation of Primary Rat Hepatocytes with Multiparameter Perfusion Control
 
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초구조적으로 보존된 세포 내 막 단백질 국소화의 전자 현미경 분석을 위한 CryoAPEX 방법

Article DOI: 10.3791/60677-v • 11:45 min • February 27th, 2020
February 27th, 2020 •
1, 2, 2,3,4, 1,4,5,6
1Department of Biological Sciences, Purdue University, 2Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, 3Purdue Institute of Inflammation, Immunology and Infectious Disease, Purdue University, 4Bindley Biosciences Center, Purdue University, 5Purdue Institute of Integrative Neuroscience, Purdue University, 6Purdue Center for Cancer Research, Purdue University

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이 프로토콜은 APEX2 태그가 달린 막 단백질이 최적으로 보존된 세포 초음파 구조 내에서 전송 전자 현미경검사법에 의해 국한될 수 있는 cryoAPEX 방법을 설명합니다.

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생물학 문제 156 CryoAPEX 막 단백질 APEX2 전송 전자 현미경 저온 방지 고압 동결 동결 대체
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