Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Detección de especies totales reactivas de…
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content.

JoVE Journal Biochemistry
Detección de especies totales reactivas de oxígeno en células adherentes por 2',7'-Dichlorodihydrofluorescein Diacetate Staining
Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays
Journal (Biochemistry)
Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays
Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry
Journal (Biochemistry)
Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry
 
Click here for the English version

Detección de especies totales reactivas de oxígeno en células adherentes por 2',7'-Dichlorodihydrofluorescein Diacetate Staining

Article DOI: 10.3791/60682-v • 04:53 min • June 23rd, 2020
June 23rd, 2020 •
1, 1
1Department of Biochemistry and Molecular Biology, University of New Mexico

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Aquí, presentamos un protocolo para detectar especies totales de oxígeno reactivo celular (ROS) usando 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA). Este método puede visualizar la localización de ROS celular en células adherentes con un microscopio de fluorescencia y cuantificar la intensidad ROS con un lector de placas de fluorescencia. Este protocolo es simple, eficiente y rentable.

Tags

Bioquímica Número 160 estrés oxidativo 2',7'-Dichlorodihydrofluorescein diacetate especies reactivas de oxígeno células adherentes microscopio de fluorescencia lector de placas de fluorescencia
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter