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JoVE Journal Developmental Biology
Isolamento, cultura e indução adipogênica de células-tronco originais de adipose da crista neural do tecido adipogênico periaorótico
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Isolamento, cultura e indução adipogênica de células-tronco originais de adipose da crista neural do tecido adipogênico periaorótico

Article DOI: 10.3791/60691-v • 08:31 min • March 2nd, 2020
March 2nd, 2020 •
*1, *2, 3, 3, 4, 5, 4, 5, 1
1Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, 2Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 3Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 4Department of Cardiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 5Department of Cardiology, Shanghai Key Laboratory of Clinical Geriatric Medicine, Huadong Hospital Affiliated to Fudan University
* These authors contributed equally

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Apresentamos um protocolo para o isolamento, cultura e indução adipogênica de células-tronco derivadas de adipose derivados da crista neural (NCADSCs) do tecido periártico adiposo de Wnt-1 Cre+/-; Rostou rosa26RFP/+. Os NCADSCs podem ser uma fonte de ADSCs facilmente acessível para modelar adipogênese ou lipogênese in vitro.

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Biologia do Desenvolvimento Questão 157 célula da crista neural Wnt-1 rato tecido adippose periaorótico células estrumárias derivadas de adipose fração vascular estrôtera cultura celular indução adipogênica
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