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Developmental Biology

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JoVE Journal Developmental Biology
Aislamiento, cultivo e inducción adipogénica de células madre originales adiposas de raíz de la cresta neuronal del tejido adiposorio
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Aislamiento, cultivo e inducción adipogénica de células madre originales adiposas de raíz de la cresta neuronal del tejido adiposorio

Article DOI: 10.3791/60691-v • 08:31 min • March 2nd, 2020
March 2nd, 2020 •
*1, *2, 3, 3, 4, 5, 4, 5, 1
1Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, 2Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 3Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 4Department of Cardiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 5Department of Cardiology, Shanghai Key Laboratory of Clinical Geriatric Medicine, Huadong Hospital Affiliated to Fudan University
* These authors contributed equally

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Presentamos un protocolo para el aislamiento, el cultivo y la inducción adipogénica de células madre derivadas de cresta neural derivadas de la cresta adiposa (NCADSC) del tejido adiposorio periaortico de Wnt-1 Cre+/-; Ratones Rosa26RFP/+. Los NCADSC pueden ser una fuente de aXenatos de fácil acceso para modelar adipogénesis o lipogénesis in vitro.

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Biología del desarrollo Número 157 célula de cresta neural Wnt-1 ratón tejido adiposorio periaortico células estromales derivadas de adippos fracción vascular estromales cultivo celular inducción adipogénica
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