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Journal / Genetics / 用骨肉瘤衍生细胞外囊泡处理的美相干细胞中的LINE-1甲基化分析…
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Genetics
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JoVE Journal Genetics
用骨肉瘤衍生细胞外囊泡处理的美相干细胞中的LINE-1甲基化分析
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用骨肉瘤衍生细胞外囊泡处理的美相干细胞中的LINE-1甲基化分析

Article DOI: 10.3791/60705-v • 12:18 min • February 1st, 2020
February 1st, 2020 •
*1,2, *1,2, 1,2, 1,2
1Department of Oral and Maxillofacial Diseases, University of Helsinki, 2Helsinki University Hospital
* These authors contributed equally

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这里描述的是使用甲基化特异性探针扩增方法分析使用骨肉瘤衍生的细胞外囊泡治疗的中位体干细胞中LINE-1元素的甲基化水平。也演示了超中心化，一种从胎儿牛血清中分离细胞外囊泡的常用程序。

Tags

遗传学， 问题 156， DNA 甲基化， LINE-1， 表观遗传学， 细胞外囊泡， EV耗尽的胎儿牛血清， 骨肉瘤
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