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Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids
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Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

Article DOI: 10.3791/60709-v • 10:40 min • June 5th, 2020
June 5th, 2020 •
*1,2,3, *1,2,3, 1,2,3, *1,2,3,4, *1,2,3
1Princess Máxima Center for Pediatric Oncology, 2Department of Cancer Research, Oncode Institute, Hubrecht Institute-KNAW Utrecht, 3Cancer Genomics Center (CGC), 4Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center (UMC) Utrecht
* These authors contributed equally

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Summary
Automatic Translation

The entire 3D structure and cellular content of organoids, as well as their phenotypic resemblance to the original tissue can be captured using the single-cell resolution 3D imaging protocol described here. This protocol can be applied to a wide range of organoids varying in origin, size and shape.

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Single-cell Resolution Three-dimensional Imaging Intact Organoids Protocol Structural Complexity Mapping Identity Sulfate Distribution Quick Three-day Protocol Optimized Various Origin Sample Preparation Non-toxic Optical Clearing Step Silicon-based Mounting Method Critical Steps Visual Demonstration Recover Organoids Basement Membrane Extract 24 Well Plates Wash PBS Solution Ice-cold Recovery Solution Horizontal Shaker 1%BSA In PBS Solution Pipette Tips BSA Coating
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