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Journal / Developmental Biology / Fluorescerende calciumbeeldvorming en…
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Developmental Biology

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JoVE Journal Developmental Biology
Fluorescerende calciumbeeldvorming en daaropvolgende in situ hybridisatie voor neuronale precursorkarakterisering in Xenopus laevis
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Fluorescerende calciumbeeldvorming en daaropvolgende in situ hybridisatie voor neuronale precursorkarakterisering in Xenopus laevis

Article DOI: 10.3791/60726-v • 09:07 min • February 18th, 2020
February 18th, 2020 •
1, 2, 3, 4, 4, 5, 5, 2
1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Harvard University, 2Department of Biology, College of William and Mary, 3Harvard Medical School, Harvard University, 4Department of Bioengineering, California Institute of Technology, 5Department of Computer Science, College of William and Mary

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We presenteren een tweedelige protocol dat fluorescerende calciumbeeldvorming combineert met in situ hybridisatie, waardoor de experimentator patronen van calciumactiviteit kan correleren met genexpressieprofielen op een eencellend niveau.

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Ontwikkelingsbiologie nummer 156 Ca2+ calcium calciumbeeldvorming calciumactiviteit neurale ontwikkeling embryonale ontwikkeling neurotransmitter neurowetenschappen
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