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JoVE Journal
Developmental Biology

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Generation af Mosaic Mammary Organoids af Differential Trypsinization
 
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Generation af Mosaic Mammary Organoids af Differential Trypsinization

Article DOI: 10.3791/60742-v 09:40 min March 11th, 2020
March 11th, 2020

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Summary

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Mælkekirtlen er en tolaget struktur, bestående af ydre myoepithelial og indre luminale epitelceller. Præsenteret er en protokol til at forberede organoids ved hjælp af differentiale trypsinization. Denne effektive metode gør det muligt for forskere at separat manipulere disse to celletyper til at udforske spørgsmål vedrørende deres roller i mælkekirtlen form og funktion.

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Udviklingsbiologi mamma bryst organoid luminal basal myoepithelial epitel 3D-kultur
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