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Journal / Biochemistry / Strategisk screening og karakterisering av Visual …
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Biochemistry

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JoVE Journal Biochemistry
Strategisk screening og karakterisering av Visual GPCR-mini-G Protein Signaling Complex for vellykket krystallisering
Assaying Protein Kinase Activity with Radiolabeled ATP
Journal (Biochemistry)
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Journal (Biochemistry)
Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
 
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Strategisk screening og karakterisering av Visual GPCR-mini-G Protein Signaling Complex for vellykket krystallisering

Article DOI: 10.3791/60747-v • 09:19 min • March 16th, 2020
March 16th, 2020 •
1,2, 1, 3, 4, 4, 4, 1
1Laboratory of Biomolecular Research, Paul Scherrer Institute, 2Department of Biology, ETH Zürich, 3Center for Radiopharmaceutical Sciences, Paul Scherrer Institute, 4Laboratory of Molecular Biology, Medical Research Council

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Denne rapporten beskriver screening av ulike vaskemidler for å forberede den visuelle GPCR, rhodopsin, og dens kompleks med mini-Go. Biokjemiske metoder som karakteriserer kvaliteten på komplekset på forskjellige stadier under rensing er demonstrert. Denne protokollen kan generaliseres til andre membranproteinkomplekser for deres fremtidige strukturelle studier.

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Biokjemi Utgave 157 vaskemiddel rensing rhodopsin-mini-Go kompleks stabilitet glykosylering retinal SEC UV-VIS spektroskopi SDS-PAGE LC-MS
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