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JoVE Journal Bioengineering
Vekst av menneske- og sauehornhinne endotelcellelag på biomaterialmembraner
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Vekst av menneske- og sauehornhinne endotelcellelag på biomaterialmembraner

Article DOI: 10.3791/60762-v • 05:20 min • February 6th, 2020
February 6th, 2020 •
1, 2, 1, 1,3,4,5,6,7,8, 1,3,4
1Queensland Eye Institute, 2King Faisal University, 3School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, 4Institute of Health and Biomedical Innovation, Queensland University of Technology, 5Science and Engineering Faculty, Queensland University of Technology, 6Faculty of Medicine, University of Queensland, 7Australian Institute for Bioengineering and Nanotechnology, University of Queensland, 8Faculty of Science, University of Western Australia

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Denne protokollen beskriver de kritiske trinnene som kreves for å etablere og vokse hornhinne endotelcellekulturer fra explants av menneskelig eller sauvev. En metode for å subculturing hornhinne endotelceller på membranøse biomaterialer er også presentert.

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Bioengineering Utgave 156 hornhinneendotel hornhinnen Descemets membran explant biomaterialmembran kulturkammer
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