Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

This content is Open Access.

量化和站点本地化两个翻译后修改的同步亲和力丰富
 
Click here for the English version

量化和站点本地化两个翻译后修改的同步亲和力丰富

Article DOI: 10.3791/60780-v 12:11 min February 27th, 2020
February 27th, 2020

Chapters

Summary

Please note that all translations are automatically generated.

Click here for the English version.

此工作流程描述了用于定量全球蛋白质组分析的多个蛋白质转化后修饰 (PTM) 的及时且经济高效的扩充性能。该协议利用肽级PTM富集与多个结合抗体,然后数据独立的采集质谱分析,以获得对PTM串扰的生物见解。

Tags

生物学, 问题 156, 翻译后修改, 免疫亲和力丰富, 相声, 站点定位, 质谱, 数据独立采集, 消融, 乙酰化, 渗出
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter