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JoVE Journal Bioengineering
Caracterizando mudanças conformais de molécula única fluxo de cisalhamento com microscopia de fluorescência
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Caracterizando mudanças conformais de molécula única fluxo de cisalhamento com microscopia de fluorescência

Article DOI: 10.3791/60784-v • 08:47 min • January 25th, 2020
January 25th, 2020 •
1, *2, *3, 3, 1,2, 1,4
1Department of Bioengineering, Lehigh University, 2Department of Materials Science and Engineering, Lehigh University, 3Department of Chemistry, Lehigh University, 4Department of Mechanical Engineering and Mechanics, Lehigh University
* These authors contributed equally

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Apresentamos um protocolo para imobilizar macromoléculas únicas em dispositivos microfluidos e quantificar mudanças em suas conformações fluxo de cisalhamento. Este protocolo é útil para caracterizar as propriedades biomecânicas e funcionais de biomoléculas, como proteínas e DNA, em um ambiente de fluxo.

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Bioengenharia Edição 155 fator von Willebrand DNA lambda microfluidos fluxo de cisalhamento TIRF microscopia de fluorescência confocal
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