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LarvaSPA, A Method for Mounting Drosophila Larva for Long-Term Time-Lapse Imaging
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LarvaSPA, A Method for Mounting Drosophila Larva for Long-Term Time-Lapse Imaging

Article DOI: 10.3791/60792-v • 08:55 min • February 27th, 2020
February 27th, 2020 •
1, 1
1Weill Institute for Cell and Molecular Biology and Department of Molecular Biology and Genetics, Cornell University

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This protocol describes a method for mounting Drosophila larvae to achieve longer than 10 h of uninterrupted time-lapse imaging in intact live animals. This method can be used to image many biological processes close to the larval body wall.

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LarvaSPA Drosophila Larva Long-term Time-lapse Imaging Live Imaging Temporal Resolution Spatial Resolution Peripheral Sensory Neurons Cellular Processes Larva Body Wall Larva Size Limitation Imaging Chamber PDMS Cuboids Minimal Cost Dendrite Development Dendrite Degeneration Dendritic Authorization Neurons Immobilizing Larvae Troubleshooting Section Aluminum Block Cover Slip UV Glue Handheld UV Lamp Packaging Tape
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