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Biochemistry

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JoVE Journal Biochemistry
Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn's Disease using Immunofluorescence
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Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn's Disease using Immunofluorescence

Article DOI: 10.3791/60813-v • 08:02 min • March 12th, 2020
March 12th, 2020 •
1, 2, 3, 2, 1
1Department of Cytobiochemistry, Faculty of Biology and Environmental Protection, University of Lodz, 2Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, 3Laboratory of Microscopic Imaging and Specialized Biological Techniques, Faculty of Biology and Environmental Protection, University of Lodz

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Summary
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The protocol presents a complete validated TNBS-induced murine model of Crohn's disease and methods for visualization of estrogen receptors by immunohistochemistry using immunofluorescence of formalin-fixed colon sections embedded in paraffin.

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Visualization Estrogen Receptors Colons Mice TNBS-induced Crohn's Disease Immunofluorescence Immunohistochemistry Protocol Dr. Sylwia Michlewska Laboratory Of Microscopic Imaging University Of Lodz Petri Dish Histological Cassette Paraformaldehyde Tissue Processor Ethanol Xylene Liquid Paraffin Metal Mold
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