Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Genetics

A subscription to JoVE is required to view this content.

使用小毛针(sh)RNA在C2C12霉细胞细胞系中编码细胞外基质蛋白的基因稳定击倒
 
Click here for the English version

使用小毛针(sh)RNA在C2C12霉细胞细胞系中编码细胞外基质蛋白的基因稳定击倒

Article DOI: 10.3791/60824-v 12:19 min February 12th, 2020
February 12th, 2020

Chapters

Summary

Please note that all translations are automatically generated.

Click here for the English version.

我们提供一种协议,使用小发夹(sh)RNA在C2C12表细胞细胞细胞中稳稳地敲除编码细胞外基质(ECM)蛋白质的基因。以 ADAMTSL2 为例,我们描述了在 C2C12 霉细胞到霉细胞分化期间,mRNA、蛋白质和细胞水平的敲除效率验证方法。

Tags

遗传学, 问题 156, ADAMTS 蛋白酶, ADAMTS样蛋白质, C2C12 肌细胞, 细胞外基质, 骨骼肌分化, 肌发生, shRNA
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter