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JoVE Journal Genetics
Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA
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Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA

Article DOI: 10.3791/60824-v • 12:19 min • February 12th, 2020
February 12th, 2020 •
1, 1, 1
1Orthopaedic Research Laboratories, Leni & Peter W. May Department of Orthopaedics, Icahn School of Medicine at Mount Sinai

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We bieden een protocol om genen die extracellulaire matrix (ECM) eiwitten coderen in C2C12 myoblasten neer te halen met behulp van small-hairpin (sh) RNA. Gericht op ADAMTSL2 als voorbeeld, beschrijven we de methoden voor de validatie van de knockdown-efficiëntie op het mRNA-, eiwit- en cellulair niveau tijdens C2C12 myoblast tot myotubedifferentiatie.

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Genetica ADAMTS proteases ADAMTS-achtige eiwitten C2C12 myoblasten extracellulaire matrix skeletspierdifferentiatie myogenese shRNA
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