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JoVE Journal
Genetics

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Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh))RNA Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA Stable
 
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Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh))RNA Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA Stable

Article DOI: 10.3791/60824-v 12:19 min February 12th, 2020
February 12th, 2020

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Summary

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Nous fournissons un protocole pour abattre de façon stable les gènes codant les protéines de matrice extracellulaire (ECM) dans les myoblastes C2C12 à l’aide d’ARN à épingle sh. Ciblant ADAMTSL2 à titre d’exemple, nous décrivons les méthodes de validation de l’efficacité de knockdown sur l’ARNm, la protéine et le niveau cellulaire pendant le myoblaste C2C12 à la différenciation myotube.

Tags

Génétique Numéro 156 protéases ADAMTS protéines ADAMTS-like myoblastes C2C12 matrice extracellulaire différenciation des muscles squelettiques myogenèse shRNA
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