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Journal / Genetics / Stabil nedsättning av gener kodning…
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JoVE Journal Genetics
Stabil nedsättning av gener kodning extracellulära Matrix Proteiner i C2C12 Myoblast Cell Line med små hårnål (sh)RNA
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Stabil nedsättning av gener kodning extracellulära Matrix Proteiner i C2C12 Myoblast Cell Line med små hårnål (sh)RNA

Article DOI: 10.3791/60824-v • 12:19 min • February 12th, 2020
February 12th, 2020 •
1, 1, 1
1Orthopaedic Research Laboratories, Leni & Peter W. May Department of Orthopaedics, Icahn School of Medicine at Mount Sinai

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Vi tillhandahåller ett protokoll för att stabilt slå ner gener som kodar extracellulära matrisproteiner (ECM) i C2C12-myoblaster med små hårnål (sh) RNA. Med inriktning på ADAMTSL2 som exempel beskriver vi metoderna för validering av knockdown-effektiviteten på mRNA-, protein- och cellnivå under C2C12-myoblast till myotubedifferentiering.

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Genetik ADAMTS proteaser ADAMTS-liknande proteiner C2C12 myoblaster extracellulär matris skelettmuskulaturdifferentiering myogenesis shRNA
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