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Establishing In Situ Closed Circuit Perfusion of Lower Abdominal Organs and Hind Limbs in Mice
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Establishing In Situ Closed Circuit Perfusion of Lower Abdominal Organs and Hind Limbs in Mice

Article DOI: 10.3791/60847-v • 05:27 min • August 13th, 2020
August 13th, 2020 •
1,2,3, 2,4, 2, 2, *2,4,5, *2
1Department of Thoracic Surgery, The First Hospital of Jilin University, 2The Skaggs School of Pharmacy and Pharmaceutical Sciences, Department of Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, 3Key Laboratory of Zoonoses Research, Ministry of Education, Jilin University, 4Department of Cardiology, China-Japan Union Hospital of Jilin University, 5Department of Gastrointestinal, Colorectal and Anal Surgery, China-Japan Union Hospital of Jilin University
* These authors contributed equally

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Summary
Automatic Translation

A protocol is described for in situ perfusion of the mouse lower body, including the bladder, the prostate, sex organs, bone, muscle and foot skin.

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In Situ Closed Circuit Perfusion Lower Abdominal Organs Hind Limbs Mice Drug Delivery Immune Interactions Ex-vivo Methods Surgery Circulating Water Bath Tubing Bubble Trap Catheterization Procedure Anesthetized Bulb See Mouse Supine Position Styrofoam Board Abdomen Incision Electro Coagulation Stomach Jejunum Colon Abdominal Aorta Vena Cava Common Iliac Artery And Vein Dissection Microscope 4-O Silk Sutures
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