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Biochemistry

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JoVE Journal Biochemistry
Umålrettet flydende kromatografi-massespektrometri-baserede metabolomiske analyse af hvedekorn
A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro
Journal (Biochemistry)
A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro
Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
Journal (Biochemistry)
Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
 
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Umålrettet flydende kromatografi-massespektrometri-baserede metabolomiske analyse af hvedekorn

Article DOI: 10.3791/60851-v • 07:10 min • March 13th, 2020
March 13th, 2020 •
1,2,3, 1,4, 5,6, 1
1Research and Innovation, Murdoch University, 2Centre for Digital Agriculture, Curtin University, 3Centre for Integrative Metabolomics and Computational Biology, School of Science, Edith Cowan University, 4Centre for Computational and System Medicine, Health Futures Institute, Murdoch University, 5Department of Primary Industries and Regional Development, 6State Agricultural Biotechnology Centre, Murdoch University

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En metode til ikke-målrettet analyse af hvede korn metabolitter og lipider præsenteres. Protokollen indeholder en acetonitril metabolit ekstraktionsmetode og omvendt fase flydende kromatografi-masse spektrometri metode, med erhvervelse i positive og negative elektrospray ionisering tilstande.

Tags

Biokemi hvede markdyrket hvede hvedesort hvedekorn landbrug metabolomik ikke-målrettet metabolomik flydende kromatografi massespektrometri
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