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Neuroscience

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JoVE Journal Neuroscience
Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion
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Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

Article DOI: 10.3791/60939-v • 09:09 min • April 12th, 2020
April 12th, 2020 •
1,2,4, 1,2,4, 1,3, 1,2,4, 3, 1,2,4, 1,2,4
1Dept. of Neurosurgery, University of Michigan Medical School, 2Dept. of Cell and Developmental Biology, University of Michigan Medical School, 3Dept. of Ophthalmology & Visual Science, University of Michigan Medical School, 4Rogel Cancer Center, University of Michigan Medical School

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Laser microdissectie (LMD) is een gevoelige en zeer reproduceerbare techniek die kan worden gebruikt om paden die glioom heterogeniteit en invasie te bemiddelen blootleggen. Hier beschrijven we een geoptimaliseerd protocol om discrete gebieden te isoleren van glioomweefsel met behulp van laser LMD, gevolgd door transcriptomische analyse.

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Neurowetenschap Nummer 158 glioom lasermicrodissectie transcriptomic analyse RNA integriteit tumor heterogeniteit invasie moleculaire doelen
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