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Journal / Genetics / Identification of Enhancer-Promoter Contacts in…
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Genetics

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JoVE Journal Genetics
Identification of Enhancer-Promoter Contacts in Embryoid Bodies by Quantitative Chromosome Conformation Capture (4C)
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Identification of Enhancer-Promoter Contacts in Embryoid Bodies by Quantitative Chromosome Conformation Capture (4C)

Article DOI: 10.3791/60960-v • 10:02 min • April 29th, 2020
April 29th, 2020 •
1,2,3, 1,2, 1,2, 1,2
1Center for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, 2Universitat Pompeu Fabra, 3Vall d'Hebron Institute of Oncology

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Summary
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We report the application of quantitative chromosome conformation capture followed by high-throughput sequencing in embryoid bodies generated from embryonic stem cells. This technique allows to identify and quantitate the contacts between putative enhancers and promoter regions of a given gene during embryonic stem cell differentiation.

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Enhancer-Promoter Contacts Quantitative Chromosome Conformation Capture (4C) Cis-regulatory Elements Chromatin Contacts Interaction Frequencies Regulatory Elements Embryoid Bodies Mouse Embryonic Stem Cells Cell Detachment Medium EB Differentiation Medium Single-cell Suspension Cell Pellet
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