JoVE Monthly Highlights: April 2018

1JoVE Content Production

Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors

Martin R. Silic1, GuangJun Zhang1,2

Here, we show the process of creating a cellular electric voltage reporter zebrafish line to visualize embryonic development, movement, and fish tumor cells in vivo.

Radiation Planning Assistant - A Streamlined, Fully Automated Radiotherapy Treatment Planning System

Laurence E. Court1, Kelly Kisling1, Rachel McCarroll1, Lifei Zhang1, Jinzhong Yang1, Hannah Simonds2, Monique du Toit2, Chris Trauernicht2, Hester Burger3, Jeannette Parkes3, Mike Mejia4, Maureen Bojador4, Peter Balter1, Daniela Branco1, Angela Steinmann1, Garrett Baltz1, Skylar Gay1, Brian Anderson1, Carlos Cardenas1, Anuja Jhingran5, Simona Shaitelman5, Oliver Bogler6, Kathleen Schmeller7, David Followill1, Rebecca Howell1, Christopher Nelson1, Christine Peterson8, Beth Beadle5,9

Radiation therapy is a highly complex cancer treatment that requires multiple specialists to create a treatment plan and provide quality assurance (QA) prior to delivery to a patient. This protocol describes the use of a fully automated system, the Radiation Planning Assistant (RPA), to create high-quality radiation treatment plans.

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Allison A. Dilliott1,2, Sali M.K. Farhan3, Mahdi Ghani4, Christine Sato4, Eric Liang5, Ming Zhang4, Adam D. McIntyre1, Henian Cao1, Lemuel Racacho6,7, John F. Robinson1, Michael J. Strong1,8, Mario Masellis9,10, Dennis E. Bulman6,7, Ekaterina Rogaeva4, Anthony Lang10,11, Carmela Tartaglia4,10, Elizabeth Finger12,13, Lorne Zinman9, John Turnbull14, Morris Freedman10,15, Rick Swartz9, Sandra E. Black9,16, Robert A. Hegele1,2

Targeted next-generation sequencing is a time- and cost-efficient approach that is becoming increasingly popular in both disease research and clinical diagnostics. The protocol described here presents the complex workflow required for sequencing and the bioinformatics process used to identify genetic variants that contribute to disease.

An In Vivo Murine Sciatic Nerve Model of Perineural Invasion

Sylvie Deborde*1, Yasong Yu*1, Andrea Marcadis1, Chun-Hao Chen1, Ning Fan2, Richard L. Bakst3, Richard J. Wong1
* These authors contributed equally

We describe an in vivo murine model of perineural invasion by injecting syngeneic pancreatic cancer cells into the sciatic nerve. The model allows for quantification of the extent of nerve invasion, and supports investigation of the cellular and molecular mechanisms of perineural invasion.