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Journal / Immunology and Infection / Omvendt transkripsjon Loop-Mediert Isothermal…
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Immunology and Infection
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JoVE Journal Immunology and Infection
Omvendt transkripsjon Loop-Mediert Isothermal Amplification (RT-LAMP) Analyse for spesifikk og rask deteksjon av Tilapia Lake Virus
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Omvendt transkripsjon Loop-Mediert Isothermal Amplification (RT-LAMP) Analyse for spesifikk og rask deteksjon av Tilapia Lake Virus

Article DOI: 10.3791/61025-v • 07:47 min • May 18th, 2020
May 18th, 2020 •
1, 2,3, 2, 4, 2,3
1Department of Biotechnology, Faculty of Applied Science, King Mongkut's University of Technology North Bangkok (KMUTNB), 2Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, 3Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies, Kasetsart University, 4Department of Chemical and Process Engineering, The Sirindhorn Thai-German International Graduate School of Engineering (TGGS), King Mongkut's University of Technology North Bangkok (KMUTNB)

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Vi presenterer en RT-LAMP analyse for påvisning av TiLV i tilapia fisk ved hjelp av enkle instrumenter over en relativt kort periode sammenlignet med konvensjonelle RT-PCR teknikker. Denne protokollen kan bidra til å kontrollere epidemispredningen av TiLVD, spesielt i utviklingsland.

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Immunologi og infeksjon Utgave 159 tilapia innsjø virus diagnose tilapia RT-LAMP RT-qPCR screening verktøy
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