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Journal / Immunology and Infection / Omvänd transkription Loop-medierad isotermisk…
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Immunology and Infection
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JoVE Journal Immunology and Infection
Omvänd transkription Loop-medierad isotermisk förstärkning (RT-LAMP) analys för specifik och snabb detektion av Tilapia Lake Virus
Imaging Leukocyte Adhesion to the Vascular Endothelium at High Intraluminal Pressure
Journal (Immunology and Infection)
Imaging Leukocyte Adhesion to the Vascular Endothelium at High Intraluminal Pressure
Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins
Journal (Immunology and Infection)
Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins
 
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Omvänd transkription Loop-medierad isotermisk förstärkning (RT-LAMP) analys för specifik och snabb detektion av Tilapia Lake Virus

Article DOI: 10.3791/61025-v • 07:47 min • May 18th, 2020
May 18th, 2020 •
1, 2,3, 2, 4, 2,3
1Department of Biotechnology, Faculty of Applied Science, King Mongkut's University of Technology North Bangkok (KMUTNB), 2Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, 3Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies, Kasetsart University, 4Department of Chemical and Process Engineering, The Sirindhorn Thai-German International Graduate School of Engineering (TGGS), King Mongkut's University of Technology North Bangkok (KMUTNB)

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Vi presenterar en RT-LAMP-analys för detektering av TiLV i tilapiafisk med enkla instrument under en relativt kort tidsperiod jämfört med konventionella RT-PCR-tekniker. Detta protokoll kan bidra till att kontrollera den epidemiska spridningen av TiLVD, särskilt i utvecklingsländerna.

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Immunologi och infektion tilapia lake virus diagnos tilapia RT-LAMP RT-qPCR screening verktyg
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