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Journal / Immunology and Infection / Tilapia Lake Virüs Ünün Özel ve Hızlı Tespiti…
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Tilapia Lake Virüs Ünün Özel ve Hızlı Tespiti İçin Ters Transkripsiyon Döngü Aracılı İzomal Amplifikasyon (RT-LAMP) Testi
Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation
Journal (Immunology and Infection)
Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation
Processing of Bronchoalveolar Lavage Fluid and Matched Blood for Alveolar Macrophage and CD4+ T-cell Immunophenotyping and HIV Reservoir Assessment
Journal (Immunology and Infection)
Processing of Bronchoalveolar Lavage Fluid and Matched Blood for Alveolar Macrophage and CD4+ T-cell Immunophenotyping and HIV Reservoir Assessment
 
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Tilapia Lake Virüs Ünün Özel ve Hızlı Tespiti İçin Ters Transkripsiyon Döngü Aracılı İzomal Amplifikasyon (RT-LAMP) Testi

Article DOI: 10.3791/61025-v • 07:47 min • May 18th, 2020
May 18th, 2020 •
1, 2,3, 2, 4, 2,3
1Department of Biotechnology, Faculty of Applied Science, King Mongkut's University of Technology North Bangkok (KMUTNB), 2Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, 3Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies, Kasetsart University, 4Department of Chemical and Process Engineering, The Sirindhorn Thai-German International Graduate School of Engineering (TGGS), King Mongkut's University of Technology North Bangkok (KMUTNB)

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Geleneksel RT-PCR tekniklerine göre nispeten kısa bir süre boyunca basit aletler kullanarak tilapia balıklarında TiLV tespiti için bir RT-LAMP tayini salıyoruz. Bu protokol, özellikle gelişmekte olan ülkelerde TiLVD'nin salgınının kontrol altına alabiliyor.

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İmmünoloji ve Enfeksiyon Sayı 159 tilapia lake virüs tanı tilapia RT-LAMP RT-qPCR tarama aracı
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